Quantitative real-time RT-PCR data analysis: current concepts and the novel “gene expression’s CT difference” formula

@article{Schefe2006QuantitativeRR,
  title={Quantitative real-time RT-PCR data analysis: current concepts and the novel “gene expression’s CT difference” formula},
  author={Jan H. Schefe and Kerstin E. Lehmann and Ivo R. Buschmann and Thomas Unger and Heiko Funke-Kaiser},
  journal={Journal of Molecular Medicine},
  year={2006},
  volume={84},
  pages={901-910}
}
For quantification of gene-specific mRNA, quantitative real-time RT-PCR has become one of the most frequently used methods over the last few years. This article focuses on the issue of real-time PCR data analysis and its mathematical background, offering a general concept for efficient, fast and precise data analysis superior to the commonly used comparative CT (ΔΔCT) and the standard curve method, as it considers individual amplification efficiencies for every PCR. This concept is based on a… Expand
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