Quantitative real-time PCR eliminates false-positives in colony screening PCR.

@article{Skarratt2014QuantitativeRP,
  title={Quantitative real-time PCR eliminates false-positives in colony screening PCR.},
  author={Kristen K. Skarratt and Stephen J. Fuller},
  journal={Journal of microbiological methods},
  year={2014},
  volume={96},
  pages={99-100}
}
We report an alternative approach to colony screening using real-time PCR (qPCR) which can be used instead of the traditional end-point PCR to eliminate false-positives and reduce processing times. False-positive transformants can easily be distinguished from true-positives by comparing Ct values derived from qPCR amplification curves. In addition, the use… CONTINUE READING