Quantitative real-time PCR assay for detection of Paenibacillus polymyxa using membrane-fusion protein-based primers.

Abstract

Paenibacillus polymyxa is known to be a plant-growthpromoting rhizobacterium. The present study describes a quantitative polymerase chain reaction (qPCR) assay for the specific detection and quantitation of P. polymyxa using a primer pair based on the sequence of a membranefusion protein for the amplification of a 268 bp DNA fragment. This study reports… (More)

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@article{Cho2012QuantitativeRP, title={Quantitative real-time PCR assay for detection of Paenibacillus polymyxa using membrane-fusion protein-based primers.}, author={Min Seok Cho and Dong Suk Park and Jung Won Lee and Hee Youn Chi and Soo-In Sohn and Bong-Kyun Jeon and Jong-Beom Ma}, journal={Journal of microbiology and biotechnology}, year={2012}, volume={22 11}, pages={1575-9} }