Quantitative proteomics using SILAC: Principles, applications, and developments.

@article{Chen2015QuantitativePU,
  title={Quantitative proteomics using SILAC: Principles, applications, and developments.},
  author={Xiulan Chen and Shasha Wei and Yanlong Ji and Xiaojing Guo and Fuquan Yang},
  journal={Proteomics},
  year={2015},
  volume={15 18},
  pages={
          3175-92
        }
}
SILAC is based on direct addition of selected stable isotope amino acids into the cell culture medium, allowing superior quantitative analysis of the cellular proteome compared to other labeling methods. The great advantages of SILAC lie in its straight-forward implementation, quantitative accuracy, and reproducibility over chemical labeling or label-free quantification strategies, favoring its adoption for proteomic research. SILAC has been widely applied to characterize the proteomic changes… CONTINUE READING
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