Quantitative proteome analysis using isotope-coded affinity tags and mass spectrometry

@article{Shiio2006QuantitativePA,
  title={Quantitative proteome analysis using isotope-coded affinity tags and mass spectrometry},
  author={Yuzuru Shiio and Ruedi Aebersold},
  journal={Nature Protocols},
  year={2006},
  volume={1},
  pages={139-145}
}
A main objective of proteomics research is to systematically identify and quantify proteins in a given proteome (cells, subcellular fractions, protein complexes, tissues or body fluids). Protein labeling with isotope-coded affinity tags (ICAT) followed by tandem mass spectrometry allows sequence identification and accurate quantification of proteins in complex mixtures, and has been applied to the analysis of global protein expression changes, protein changes in subcellular fractions… CONTINUE READING