Quantitative phosphoproteomics reveals the role of the AMPK plant ortholog SnRK1 as a metabolic master regulator under energy deprivation

@inproceedings{Nukarinen2016QuantitativePR,
  title={Quantitative phosphoproteomics reveals the role of the AMPK plant ortholog SnRK1 as a metabolic master regulator under energy deprivation},
  author={Ella Nukarinen and Thomas N{\"a}gele and Lorenzo Pedrotti and Bernhard Wurzinger and Andrea Mair and Ramona Landgraf and Frederik B{\"o}rnke and Johannes Hanson and Markus Teige and Elena Baena-Gonz{\'a}lez and Wolfgang Dr{\"o}ge-Laser and Wolfram Weckwerth},
  booktitle={Scientific reports},
  year={2016}
}
Since years, research on SnRK1, the major cellular energy sensor in plants, has tried to define its role in energy signalling. However, these attempts were notoriously hampered by the lethality of a complete knockout of SnRK1. Therefore, we generated an inducible amiRNA::SnRK1α2 in a snrk1α1 knock out background (snrk1α1/α2) to abolish SnRK1 activity to understand major systemic functions of SnRK1 signalling under energy deprivation triggered by extended night treatment. We analysed the in vivo… CONTINUE READING
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In Plant Proteomics: Methods and Protocols

  • G.J.M. Beckers, W. Hoehenwarter, H. Röhrig, U. Conrath, W. Weckwerth
  • 2014

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