Quantitative phenotypic analysis of yeast deletion mutants using a highly parallel molecular bar–coding strategy

@article{Shoemaker1996QuantitativePA,
  title={Quantitative phenotypic analysis of yeast deletion mutants using a highly parallel molecular bar–coding strategy},
  author={D. DeWayne Shoemaker and Deval A. Lashkari and Don H. Morris and Mike Mittmann and Ronald W. Davis},
  journal={Nature Genetics},
  year={1996},
  volume={14},
  pages={450-456}
}
A quantitative and highly parallel method for analysing deletion mutants has been developed to aid in determining the biological function of thousands of newly identified open reading frames (ORFs) in Saccharomyces cerevisiae. This approach uses a PCR targeting strategy to generate large numbers of deletion strains. Each deletion strain is labelled with a unique 20–base tag sequence that can be detected by hybridization to a high–density oligonucleotide array. The tags serve as unique… CONTINUE READING

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