Quantitative multiplex methylation-specific PCR assay for the detection of promoter hypermethylation in multiple genes in breast cancer.

@article{Fackler2004QuantitativeMM,
  title={Quantitative multiplex methylation-specific PCR assay for the detection of promoter hypermethylation in multiple genes in breast cancer.},
  author={Mary Jo Fackler and Megan McVeigh and Jyoti Mehrotra and Marissa A. Blum and J. J. Harlaar G. H. van Ramshorst J. Nieuwenhuizen J. Lange and Amanda Lapides and Elizabeth Garrett and Pedram Argani and Saraswati V. Sukumar},
  journal={Cancer research},
  year={2004},
  volume={64 13},
  pages={4442-52}
}
If detected early, breast cancer is eminently curable. To detect breast cancer in samples with little cellularity, a high level of sensitivity is needed. Tumor-specific promoter hypermethylation has provided such a valuable tool for detection of cancer cells in biological samples. To accurately assess promoter hypermethylation for many genes simultaneously in small samples, we developed a novel method, quantitative multiplex-methylation-specific PCR (QM-MSP). QM-MSP is highly sensitive (1 in 10… CONTINUE READING