Quantitative monitoring of pluripotency gene activation after somatic cloning in cattle.

Abstract

The development of somatic cell nuclear transfer (SCNT) embryos critically depends on appropriate reprogramming and expression of pluripotency genes, such as Pou5f1/POU5F1 (previously known as Oct4/OCT4). To study POU5F1 transcription activation in living bovine SCNT embryos without interference by maternal POU5F1 mRNA, we generated chromosomally normal… (More)

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@article{Wuensch2007QuantitativeMO, title={Quantitative monitoring of pluripotency gene activation after somatic cloning in cattle.}, author={Annegret Wuensch and Felix Andreas Habermann and Satoshi Kurosaka and Regina Klose and Valeri Zakhartchenko and H. Graf von Reichenbach and Fred Sinowatz and K. John McLaughlin and Eckhard Wolf}, journal={Biology of reproduction}, year={2007}, volume={76 6}, pages={983-91} }