Quantitative molecular phenotyping of gill remodeling in a cichlid fish responding to salinity stress.

@article{Kltz2013QuantitativeMP,
  title={Quantitative molecular phenotyping of gill remodeling in a cichlid fish responding to salinity stress.},
  author={Dietmar K{\"u}ltz and Johnathon Li and Alison M. Gardell and Romina Sacchi},
  journal={Molecular & cellular proteomics : MCP},
  year={2013},
  volume={12 12},
  pages={
          3962-75
        }
}
A two-tiered label-free quantitative (LFQ) proteomics workflow was used to elucidate how salinity affects the molecular phenotype, i.e. proteome, of gills from a cichlid fish, the euryhaline tilapia (Oreochromis mossambicus). The workflow consists of initial global profiling of relative tryptic peptide abundances in treated versus control samples followed by targeted identification (by MS/MS) and quantitation (by chromatographic peak area integration) of validated peptides for each protein of… CONTINUE READING

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