Quantitative microtiter cytotoxicity assay for Shigella toxin.

  title={Quantitative microtiter cytotoxicity assay for Shigella toxin.},
  author={M. K. Gentry and Joel M. Dalrymple},
  journal={Journal of clinical microbiology},
  volume={12 3},
The cytotoxic activity of Shigella dysenteriae 1 was assayed by exposing HeLa cells in microtiter cultures to dilutions of toxin. Exposure to toxin caused either failure of cells in suspension to attach or detachment of cells from established monolayers. Estimates of toxin potency were made by staining residual cells with crystal violet and visually inspecting the stained plates. Quantitation of the cytotoxic effect was made possible by eluting and spectrophotometrically measuring the stain… CONTINUE READING

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