Quantitative measurement of human cytokine gene expression by polymerase chain reaction.

We have developed a method in which human cytokine gene expression can be quantitated using gene amplification technology. Total RNA was extracted from a human T cell line, reverse transcribed to cDNA, and amplified using the polymerase chain reaction (PCR). Inclusion of a radiolabeled nucleotide in the PCR reaction mixture followed by electrophoresis and… (More)