Quantitative measurement of fibrosis in pancreatic tissue. Evaluation of a colorimetric method.

@article{Valderrama1991QuantitativeMO,
  title={Quantitative measurement of fibrosis in pancreatic tissue. Evaluation of a colorimetric method.},
  author={Rodrigo Valderrama and Silvia A Navarro and Eduardo Rafael Barreda del Campo and Jean J Camps and A. Gim{\'e}nez Gim{\'e}nez and Albert Pares and Juan Caballer{\'i}a},
  journal={International journal of pancreatology : official journal of the International Association of Pancreatology},
  year={1991},
  volume={10 1},
  pages={23-9}
}
A colorimetric method has been used to quantify the collagen contained in 23 specimens of pancreatic tissue (11 controls and 12 chronic pancreatitis). The method takes advantage of the selective capacity of Sirius red to stain collagen protein and of rapid green to stain noncollagen protein. The results obtained by this method were compared with those of standard morphometry to determine tissue fibrosis. With the morphometric method, the values of the control group were 6.6 +/- 4.0% (fiber area… CONTINUE READING