Quantitative measurement of biotin-binding immunoglobulin G in human sera using F(ab')2 anti-human IgG-coated multi-well plates.

@article{Muratsugu2003QuantitativeMO,
  title={Quantitative measurement of biotin-binding immunoglobulin G in human sera using F(ab')2 anti-human IgG-coated multi-well plates.},
  author={Makoto Muratsugu and Eri Muramoto and Toru Fukui},
  journal={Biological & pharmaceutical bulletin},
  year={2003},
  volume={26 11},
  pages={
          1605-8
        }
}
Biotin-binding human immunoglobulin G (B-IgG) was quantitatively measured using an F(ab')2anti-human IgG-coated multi-well microplate for the first time. B-IgG was caught by F(ab')2anti-human IgG and was detected by the following detecting reagents: Peroxidase-labeled streptavidin, avidin and peroxidase-biotin, or avidin-biotinylated peroxidase complex with method A, B, or C, respectively. Commercially available B-IgG was detected by all these three methods. However, method A and B could not… CONTINUE READING
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