Quantitative mass spectrometry reveals a role for the GTPase Rho1p in actin organization on the peroxisome membrane

@article{Marelli2004QuantitativeMS,
  title={Quantitative mass spectrometry reveals a role for the GTPase Rho1p in actin organization on the peroxisome membrane},
  author={Marcello Marelli and Jennifer J. Smith and Sunhee Jung and Eugene C Yi and Alexey I. Nesvizhskii and Rowan H. Christmas and Ramsey A. Saleem and Yuen Yi C. Tam and Andrei Fagarasanu and David R. Goodlett and Ruedi Aebersold and Richard A Rachubinski and John D. Aitchison},
  journal={The Journal of Cell Biology},
  year={2004},
  volume={167},
  pages={1099 - 1112}
}
We have combined classical subcellular fractionation with large-scale quantitative mass spectrometry to identify proteins that enrich specifically with peroxisomes of Saccharomyces cerevisiae. In two complementary experiments, isotope-coded affinity tags and tandem mass spectrometry were used to quantify the relative enrichment of proteins during the purification of peroxisomes. Mathematical modeling of the data from 306 quantified proteins led to a prioritized list of 70 candidates whose… CONTINUE READING