Quantitative interpretation of binding reactions of rapidly diffusing species using fluorescence recovery after photobleaching.

@article{Tsibidis2009QuantitativeIO,
  title={Quantitative interpretation of binding reactions of rapidly diffusing species using fluorescence recovery after photobleaching.},
  author={George Tsibidis},
  journal={Journal of microscopy},
  year={2009},
  volume={233 3},
  pages={384-90}
}
Fluorescence recovery after photobleaching (FRAP) measurements offer an important tool for analyzing diffusion and binding processes. Confocal scanning laser microscopes that are used in FRAP experiments bleach regions with a radially Gaussian distributed profile. Previous attempts to derive analytical expressions in the case of processes governed by fast diffusion have overlooked the characteristics of the instruments used to perform FRAP measurements and therefore led to approximating… CONTINUE READING

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