Quantitative insights into actin rearrangements and bacterial target site selection from Salmonella Typhimurium infection of micropatterned cells.

@article{Vonaesch2013QuantitativeII,
  title={Quantitative insights into actin rearrangements and bacterial target site selection from Salmonella Typhimurium infection of micropatterned cells.},
  author={Pascale Vonaesch and Steven Cardini and Mikael Erik Sellin and Bruno Goud and Wolf-Dietrich Hardt and Kristine Schauer},
  journal={Cellular microbiology},
  year={2013},
  volume={15 11},
  pages={1851-65}
}
Reorganization of the host cell actin cytoskeleton is crucial during pathogen invasion. We established micropatterned cells as a standardized infection model for cell invasion to quantitatively study actin rearrangements triggered by Salmonella Typhimurium (S. Tm). Micropatterns of extracellular matrix proteins force cells to adopt a reproducible shape avoiding strong cell-to-cell variations, a major limitation in classical cell culture conditions. S. Tm induced F-actin-rich ruffles and invaded… CONTINUE READING
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