Quantitative in vivo measurement of glutathione in Arabidopsis cells.

  title={Quantitative in vivo measurement of glutathione in Arabidopsis cells.},
  author={Andreas J. Meyer and Mike J. May and Mark D. Fricker},
  journal={The Plant journal : for cell and molecular biology},
  volume={27 1},
A new, non-destructive assay is described to quantify cytoplasmic glutathione (GSH) levels in vivo in single cells or populations of cells from Arabidopsis suspension cultures. Cytoplasmic GSH was labelled with monochlorobimane (MCB) in situ to give a fluorescent GSH-bimane (GSB) conjugate. At low (10-100 microM) concentrations of MCB, labelling was mediated by a glutathione S-transferase, which confers specificity for GSH. HPLC analysis of MCB-labelled low molecular-weight thiols showed that… 

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