Quantitative in vivo measurement of glutathione in Arabidopsis cells.

@article{Meyer2001QuantitativeIV,
  title={Quantitative in vivo measurement of glutathione in Arabidopsis cells.},
  author={A. J. Meyer and M. May and M. Fricker},
  journal={The Plant journal : for cell and molecular biology},
  year={2001},
  volume={27 1},
  pages={
          67-78
        }
}
  • A. J. Meyer, M. May, M. Fricker
  • Published 2001
  • Biology, Medicine
  • The Plant journal : for cell and molecular biology
  • A new, non-destructive assay is described to quantify cytoplasmic glutathione (GSH) levels in vivo in single cells or populations of cells from Arabidopsis suspension cultures. Cytoplasmic GSH was labelled with monochlorobimane (MCB) in situ to give a fluorescent GSH-bimane (GSB) conjugate. At low (10-100 microM) concentrations of MCB, labelling was mediated by a glutathione S-transferase, which confers specificity for GSH. HPLC analysis of MCB-labelled low molecular-weight thiols showed that… CONTINUE READING
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    References

    SHOWING 1-10 OF 49 REFERENCES
    Measurement of glutathione levels in intact roots of Arabidopsis
    • 68
    • PDF
    Oxidative Stimulation of Glutathione Synthesis in Arabidopsis thaliana Suspension Cultures
    • 429
    • PDF
    Kinetic analysis of glutathione in anchored cells with monochlorobimane.
    • 52
    Evidence for posttranscriptional activation of gamma-glutamylcysteine synthetase during plant stress responses.
    • 136
    • Highly Influential
    • PDF
    The use of monochlorobimane to determine hepatic GSH levels and synthesis.
    • 215
    Kinetic analysis of the intracellular conjugation of monochlorobimane by IC-21 murine macrophage glutathione-S-transferase.
    • 23
    Glutathione biosynthesis in Arabidopsis trichome cells.
    • 158
    • PDF