Quantitative in vivo and in vitro characterization of co-infection by two genetically distant grass carp reoviruses.

@article{Wang2013QuantitativeIV,
  title={Quantitative in vivo and in vitro characterization of co-infection by two genetically distant grass carp reoviruses.},
  author={Tu Wang and Jiale Li and Liqun Lu},
  journal={The Journal of general virology},
  year={2013},
  volume={94 Pt 6},
  pages={
          1301-9
        }
}
Grass carp reovirus (GCRV) is one of the most serious pathogens threatening grass carp (Ctenopharyngodon idella) production in China. Through sequence analysis, the co-existence of two genetically distant grass carp reoviruses, named GCRV-JX01 and GCRV-JX02, was revealed in the same diseased grass carp sample collected in 2011. GCRV-JX01 and GCRV-JX02 shared high levels of homology with GCRV-873 and GCRV-GD108, respectively. In contrast to GCRV-JX01, GCRV-JX02 induced no cytopathic effect in… Expand
Significant inhibition of two different genotypes of grass carp reovirus in vitro using multiple shRNAs expression vectors.
TLDR
The results in this study indicated that this multiple-shRNAs expression system could be used as a cross-reactive antiviral agent for treating the hemorrhagic disease of grass carp caused by multiple genotypes of GCRV. Expand
Fish reovirus GCReV-109 VP33 protein elicits protective immunity in rare minnows
TLDR
The death of infected fish was delayed and the mortality fell to 10 % when fish were treated with the anti-VP33 antiserum, suggesting that it might be useful for the prevention and control of fish reoviral disease. Expand
Identification, Virulence, and Molecular Characterization of a Recombinant Isolate of Grass Carp Reovirus Genotype I
TLDR
Bootscan analysis of the complete genomic sequence confirmed the hypothesis that recombination events between 873/GCHV and other GSRV-like viruses were also accompanied by gene mutations, suggesting that a recombination event had occurred. Expand
GCRV 096 VP6 protein and its impacts on GCRV replication with different genotypes in CIK cells
TLDR
The results indicated that the protein VP6 and VP6 antiserum did not provide cross-protection against GCRV strains and this can be attributed to differences among G CRV genotypes. Expand
Inhibitor analysis revealed that clathrin-mediated endocytosis is involed in cellular entry of type III grass carp reovirus
TLDR
The data have suggested that GCRV104 enters CIK cells through clathrin-mediated endocytosis in a pH-dependent manner, and it is suggested that dynamin is critical for efficient viral entry. Expand
Infection of grass carp reovirus induced the expressional suppression of pro-viral Fibulin-4 in host cells.
TLDR
Repression of Fibulin-4 expression seemed to be involved in anti-viral response in grass carp Ctenopharyngodon idella, and was inhibited upon the viral challenge in grass cap GCO cells. Expand
Disruption of clathrin-dependent trafficking results in the failure of grass carp reovirus cellular entry
TLDR
The findings suggest that GCRV might enter CIK cells via clathrin-mediated endocytosis in a pH-dependent manner and that dynamin is critical for efficient viral entry. Expand
Grass carp reovirus NS26 interacts with cellular lipopolysaccharide-induced tumor necrosis factor-alpha factor, LITAF
TLDR
In vitro and in vivo data demonstrated the interaction between cellular LITAF and GCRV NS26 and myc-NS26 colocalized with GFP-LITAF in CIK cells. Expand
Isolation, Identification, and Genomic Analysis of a Novel Reovirus from Healthy Grass Carp and Its Dynamic Proliferation In Vitro and In Vivo
TLDR
Analysis of isolate of HGCRV induced a typical cytopathic effect in fish cell lines, such as infected cell shrank, apoptosis, and plague-like syncytia, and RNA-dependent RNA-polymerase (RdRp) protein-based phylogenetic analysis showed that HG CRV clustered with Aquareovirus-C (AqRV-C) prior to joining a branch common with other aquareoviruses. Expand
Targeting Heat Shock Protein 70 as an antiviral strategy against grass carp reovirus infection.
TLDR
It is speculated that HSP70 might be a host factor in the process of GCRV infecting CIK cells, therefore, itmight be a potential antiviral target for G CRV infection. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 24 REFERENCES
Complete genomic sequence of a reovirus isolated from grass carp in China.
TLDR
Morphological and pathological analyses, and genetic characterization of GCRV-GD108 suggested that it may be a new species of AQRV and it was more closely related with ORVs than other AQRVs. Expand
Suppression of RNA interference pathway in vitro by Grass carp reovirus
TLDR
Investigation of Grass carp reovirus replication on the RNAi pathway of grass carp kidney cells indicated that replication of GCRV correlated with the increased transcription level of the Dicer gene and functional inhibition of in vitro synthesized egfp-siRNA in silencing the EGFP reporter gene. Expand
Characterization of infectious particles of grass carp reovirus by treatment with proteases
TLDR
Assays between infectivity and protein component indicated that the enhancement of infectivity was correlated to the complete digestion of the outer capsid protein VP7 and partial cleavage of VP5. Expand
Protection of grass carp, Ctenopharyngon idellus (Valenciennes), through oral administration of a subunit vaccine against reovirus.
TLDR
A mixture of VP5 and VP7 proteins was designed as a subunit vaccine candidate and developed a rapid method for the production of oral subunit vaccines for the control of GCRV infection in C. idellus. Expand
Molecular analysis of grass carp reovirus HZ08 genome segments 1–3 and 5–6
TLDR
Preliminary phylogenetic analysis using the amino acid sequences of the RNA-dependent RNA polymerases protein encoded by S2 revealed that HZ08 formed a cluster close to the aquareovirus, but was far from the other isolates, which indicated that Hz08 is likely to be a new member of Aquareov virus. Expand
Common evolutionary origin of aquareoviruses and orthoreoviruses revealed by genome characterization of Golden shiner reovirus, Grass carp reovirus, Striped bass reovirus and golden ide reovirus (genus Aquareovirus, family Reoviridae).
TLDR
Genetic analysis revealed homology in the genes of the aquareovirus and orthoreoviruses groups, indicating clear genetic relatedness between members of distinct genera, unique within the family Reoviridae. Expand
Sequence of genome segments 1, 2, and 3 of the grass carp reovirus (Genus Aquareovirus, family Reoviridae).
TLDR
The genome segments 1, 2, and 3 of the grass carp reovirus (GCRV), a tentative species assigned to genus Aquareovirus, family Reoviridae, were sequenced and revealed that VP2 is the viral polymerase, while VP1 might represent the viral guanylyl/methyl transferase and VP3 the NTPase/helicase. Expand
3D reconstruction and capsid protein characterization of grass carp reovirus
TLDR
The high structural similarities of isolated G CRV and MRV suggest that future structural studies focusing on GCRV entering into and replicating within its host cell are necessary in order to fully understand the structural basis of GCRv pathogenesis. Expand
Subnanometer-resolution structures of the grass carp reovirus core and virion.
TLDR
The results suggest that GCRV occupies a unique structure niche between the simpler cypoviruses and the considerably more complex mammalian orthoreovirus, thus providing an important model for understanding the structural and functional conservation and diversity of this enormous family of dsRNA viruses. Expand
Evaluation of cell lines and immunofluorescence and plaque assay procedures for quantifying reoviruses in sewage
TLDR
Twelve continuous cell lines were tested to determine their sensitivity to reovirus types 1, 2, and 3 isolated from sewage, and Madin-Darby bovine kidney, rhesus monkey kidney, and human embryonic intestinal cells were most sensitive. Expand
...
1
2
3
...