Quantitative improvement of 16S rDNA DGGE analysis for soil bacterial community using real-time PCR.

@article{Ahn2009QuantitativeIO,
  title={Quantitative improvement of 16S rDNA DGGE analysis for soil bacterial community using real-time PCR.},
  author={Jae-Hyung Ahn and Yoo-Jeong Kim and Taesung Kim and Hong-Gyu Song and ChulHee Kang and Jong-Ok Ka},
  journal={Journal of microbiological methods},
  year={2009},
  volume={78 2},
  pages={216-22}
}
Denaturing gradient gel electrophoresis (DGGE) of 16S rDNA fragments has been frequently used to profile a structure of the bacterial community in a given soil. However, this procedure has various types of intrinsic error and bias, thus often misleads the relative abundance of bacterial populations. In order to establish a reliability for the current 16S rDNA DGGE method, we investigated various parameters and potential sources of errors in the DGGE procedures, such as primer mismatch, dNTP… CONTINUE READING

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