Quantitative immunocytochemical assays of topoisomerase II in lung adenocarcinoma cell lines. Correlation to topoisomerase II alpha content and topoisomerase II catalytic activity.

@article{Yamazaki1996QuantitativeIA,
  title={Quantitative immunocytochemical assays of topoisomerase II in lung adenocarcinoma cell lines. Correlation to topoisomerase II alpha content and topoisomerase II catalytic activity.},
  author={Kotaro Yamazaki and Hiroshi Isobe and Toshihiko Hanada and Noriaki Sukoh and Shigeaki Ogura and Yoshikazu Kawakami},
  journal={Acta oncologica},
  year={1996},
  volume={35 4},
  pages={417-23}
}
The examination of topoisomerase II alpha content by Western blot analysis or topoisomerase II catalytic activity by decatenation of kDNA requires a large number of cells, but it is difficult to collect sufficient cells for these biochemical analyses from lung cancer patients by transbronchial brushing or aspiration. In this study, we explored the relationship between these biochemical analyses and topoisomerase II immunostaining in cytospin preparations of three lung adenocarcinoma cell lines… CONTINUE READING