Quantitative immunoassay for complexes of prostate-specific antigen with alpha2-macroglobulin.

@article{Espaa1996QuantitativeIF,
  title={Quantitative immunoassay for complexes of prostate-specific antigen with alpha2-macroglobulin.},
  author={Francisco Espa{\~n}a and J. M. S{\'a}nchez-Cuenca and Amparo Estell{\'e}s and Juan Gilabert and John H. Griffin and Mary Jo Heeb},
  journal={Clinical chemistry},
  year={1996},
  volume={42 4},
  pages={545-50}
}
We have developed two ELISAs for quantifying complexes of prostate-specific antigen (PSA) with alpha2-macroglobulin (alpha2M), using partially purified PSA:alpha2M complex as the calibrator. One ELISA was designed to evaluate )SA:alpha2M complex in fluids containing a huge excess of PSA over the amount of complex (semen-derived fluids), the other for use in fluids containing an excess of alpha2M over PSA (blood plasma). The range of the assays was 2-1000 micrograms/L for PSA complexed to… CONTINUE READING