Quantitative imaging of single, unstained viruses with coherent x rays.

  title={Quantitative imaging of single, unstained viruses with coherent x rays.},
  author={Changyong Song and Huaidong Jiang and Adrian P. Mancuso and Bagrat Amirbekian and Li Peng and Ren Sun and Sanket Shah and Z. Hong Zhou and Tetsuya Ishikawa and Jianwei (John) Miao},
  journal={Physical review letters},
  volume={101 15},
We report the recording and reconstruction of x-ray diffraction patterns from single, unstained viruses, for the first time. By separating the diffraction pattern of the virus particles from that of their surroundings, we performed quantitative and high-contrast imaging of a single virion. The structure of the viral capsid inside a virion was visualized. This work opens the door for quantitative x-ray imaging of a broad range of specimens from protein machineries and viruses to cellular… 

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