Quantitative imaging of single, unstained viruses with coherent x rays.

@article{Song2008QuantitativeIO,
  title={Quantitative imaging of single, unstained viruses with coherent x rays.},
  author={Changyong Song and Huaidong Jiang and Adrian P. Mancuso and Bagrat Amirbekian and Li Peng and Ren Sun and Sanket Shah and Z. Hong Zhou and Tetsuya Ishikawa and Jianwei (John) Miao},
  journal={Physical review letters},
  year={2008},
  volume={101 15},
  pages={
          158101
        }
}
We report the recording and reconstruction of x-ray diffraction patterns from single, unstained viruses, for the first time. By separating the diffraction pattern of the virus particles from that of their surroundings, we performed quantitative and high-contrast imaging of a single virion. The structure of the viral capsid inside a virion was visualized. This work opens the door for quantitative x-ray imaging of a broad range of specimens from protein machineries and viruses to cellular… 

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References

SHOWING 1-10 OF 40 REFERENCES
Biological imaging by soft x-ray diffraction microscopy
TLDR
This work represents a previously uncharacterized application of x-ray diffraction microscopy to a specimen of this complexity and provides confidence in the feasibility of the ultimate goal of imaging biological specimens at 10-nm resolution in three dimensions.
Imaging whole Escherichia coli bacteria by using single-particle x-ray diffraction
TLDR
The successful recording and reconstruction of diffraction patterns from biological samples reported here represent an important step toward the potential of imaging single biomolecules at near-atomic resolution by combining single-particle diffraction with x-ray free electron lasers.
Femtosecond diffractive imaging with a soft-X-ray free-electron laser
Theory predicts1,2,3,4 that, with an ultrashort and extremely bright coherent X-ray pulse, a single diffraction pattern may be recorded from a large macromolecule, a virus or a cell before the sample
Reconstruction of the shapes of gold nanocrystals using coherent x-ray diffraction.
TLDR
The successful phasing of an oversampled hard x-ray diffraction pattern measured from a single nanocrystal of gold is reported, and this has recently been demonstrated.
Quantitative image reconstruction of GaN quantum dots from oversampled diffraction intensities alone.
TLDR
It is reported here that, when the missing data are confined within the centrospeckle, theMissing data problem can be reliably solved and this work in principle clears the way for single-shot imaging experiments using x-ray free electron lasers.
Diffractive imaging of highly focused X-ray fields
The rapid development of new sources of coherent X-rays, such as third-generation synchrotrons, high-harmonic-generation lasers1 and X-ray free-electron lasers2, has led to the emergence of the new
Potential for biomolecular imaging with femtosecond X-ray pulses
TLDR
Computer simulations are used to investigate the structural information that can be recovered from the scattering of intense femtosecond X-ray pulses by single protein molecules and small assemblies and predict that ultrashort, high-intensity X-rays from free-electron lasers that are currently under development will provide a new approach to structural determinations with X- rays.
An approach to three-dimensional structures of biomolecules by using single-molecule diffraction images
We describe an approach to the high-resolution three-dimensional structural determination of macromolecules that utilizes ultrashort, intense x-ray pulses to record diffraction data in combination
Three-dimensional mapping of a deformation field inside a nanocrystal
TLDR
The method of measuring and inverting diffraction patterns from nanocrystals represents a vital step towards the ultimate goal of atomic resolution single-molecule imaging that is a prominent justification for development of X-ray free-electron lasers.
Three-Dimensional Structure of Herpes Simplex Virus from Cryo-Electron Tomography
TLDR
Herpes simplex virus, a DNA virus of high complexity, consists of a nucleocapsid surrounded by the tegument—a protein compartment—and the envelope, which was seen to form an asymmetric cap in cryo–electron tomograms of isolated virions.
...
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