Quantitative imaging of nitrogen fixation by individual bacteria within animal cells.

@article{Lechene2007QuantitativeIO,
  title={Quantitative imaging of nitrogen fixation by individual bacteria within animal cells.},
  author={Claude P. Lechene and Yvette A. Luyten and Gregory Mcmahon and Daniel L Distel},
  journal={Science},
  year={2007},
  volume={317 5844},
  pages={
          1563-6
        }
}
Biological nitrogen fixation, the conversion of atmospheric nitrogen to ammonia for biosynthesis, is exclusively performed by a few bacteria and archaea. Despite the essential importance of biological nitrogen fixation, it has been impossible to quantify the incorporation of nitrogen by individual bacteria or to map the fate of fixed nitrogen in host cells. In this study, with multi-isotope imaging mass spectrometry we directly imaged and measured nitrogen fixation by individual bacteria within… CONTINUE READING

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