Quantitative image analysis tool to study the plasma membrane localization of proteins and cortical actin in neuroendocrine cells.

BACKGROUND Adrenal chromaffin cells are a widely used model system to study regulated exocytosis and other membrane-associated processes. Alterations in the amount and localization of the proteins involved in these processes can be visualized with fluorescent probes that report the effect of different stimuli or genetic modifications. However, the… CONTINUE READING