Quantitative high-speed imaging of entire developing embryos with simultaneous multiview light-sheet microscopy

  title={Quantitative high-speed imaging of entire developing embryos with simultaneous multiview light-sheet microscopy},
  author={Raju Tomer and Khaled A. Khairy and Fernando Amat and Philipp J. Keller},
  journal={Nature Methods},
Live imaging of large biological specimens is fundamentally limited by the short optical penetration depth of light microscopes. To maximize physical coverage, we developed the SiMView technology framework for high-speed in vivo imaging, which records multiple views of the specimen simultaneously. SiMView consists of a light-sheet microscope with four synchronized optical arms, real-time electronics for long-term sCMOS-based image acquisition at 175 million voxels per second, and computational… 

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