Quantitative high-resolution melting analysis for detecting adulterations.

@article{Mader2011QuantitativeHM,
  title={Quantitative high-resolution melting analysis for detecting adulterations.},
  author={Eduard Mader and Joana Ruzicka and Corinna Schmiderer and Johannes Franz Novak},
  journal={Analytical biochemistry},
  year={2011},
  volume={409 1},
  pages={153-5}
}
Admixtures of different plant species are a common problem in raw materials for medicinal use. Two exemplary assays were developed to admixtures in Helleborus niger with high-resolution melting analysis. HRM proved to be a very sensitive tool in detecting admixtures, able to detect a ratio of 1:1000 with unknown species, and of 1:200,000 with Veratrum nigrum. The example proves the ability of HRM for quantification in multiplex PCR. The method is not limited to detecting adulterations. It can… CONTINUE READING

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A. Lorente, W. Mueller, E. Urdangarin, P. Lazcoz
von Deimling, J. Castresana, Detection of methylation in promoter sequences by melting curve analysisbased semiquantitative real time PCR, BMC Cancer 8 • 2008
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Detection of methylation in promoter sequences by melting curve analysisbased semiquantitative real time PCR

W. Mueller A. Lorente, E. Urdangarin, P. Lazcoz, A. von Deimling, J. Castresana
BMC Cancer • 2008

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