Quantitative glycomics of human whole serum glycoproteins based on the standardized protocol for liberating N-glycans.

@article{Kita2007QuantitativeGO,
  title={Quantitative glycomics of human whole serum glycoproteins based on the standardized protocol for liberating N-glycans.},
  author={Yoko Kita and Yoshiaki Miura and Jun-Ichi Furukawa and Mika Nakano and Yasuro Shinohara and Masahiro Ohno and Akio Takimoto and Shin-Ichiro Nishimura},
  journal={Molecular & cellular proteomics : MCP},
  year={2007},
  volume={6 8},
  pages={
          1437-45
        }
}
  • Yoko Kita, Yoshiaki Miura, +5 authors Shin-Ichiro Nishimura
  • Published in
    Molecular & cellular…
    2007
  • Biology, Medicine
  • Global glycomics of human whole serum glycoproteins appears to be an innovative and comprehensive approach to identify surrogate non-invasive biomarkers for various diseases. Despite the fact that quantitative glycomics is premised on highly efficient and reproducible oligosaccharide liberation from human serum glycoproteins, it should be noted that there is no validated protocol for which deglycosylation efficiency is proven to be quantitative. To establish a standard procedure to evaluate N… CONTINUE READING

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    Citations

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    BlotGlycoABCTM, an integrated glycoblotting technique for rapid and large scale clinical glycomics.

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    CITES BACKGROUND & METHODS

    HPLC-based analysis of serum N-glycans on a 96-well plate platform with dedicated database software.

    VIEW 9 EXCERPTS
    CITES METHODS
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    Reversed-phase separation methods for glycan analysis