Quantitative gene monitoring of microbial tetracycline resistance using magnetic luminescent nanoparticles.

@article{Son2010QuantitativeGM,
  title={Quantitative gene monitoring of microbial tetracycline resistance using magnetic luminescent nanoparticles.},
  author={Ahjeong Son and Ian Mason Kennedy and Kate M. Scow and Krassimira R. Hristova},
  journal={Journal of environmental monitoring : JEM},
  year={2010},
  volume={12 6},
  pages={1362-7}
}
A magnetic/luminescent nanoparticles (MLNPs) based DNA hybridization method was developed for quantitative monitoring of antibiotic resistance genes and gene-expression in environmental samples. Manipulation of magnetic field enabled the separation of the MLNPs-DNA hybrids from the solution and the fluorescence of MLNPs normalized the quantity of target DNA. In our newly developed MLNPs-DNA assay, linear standard curves (R(2) = 0.99) of target gene was determined with the detection limit of 620… CONTINUE READING
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