Quantitative fluorescence measures for determination of intracellular perforin content.

Abstract

We present methodologic details and operating characteristics of a procedure with whole blood for the quantitative assessment of intracellular perforin within distinct lymphocyte subsets. Using this method, we analyzed 20 healthy controls and 2 individuals with an inherited deficiency of perforin. The mean +/- standard deviation perforin contents of natural… (More)

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@article{Maher2002QuantitativeFM, title={Quantitative fluorescence measures for determination of intracellular perforin content.}, author={Kevin J. Maher and Nancy G. Klimas and Barry E. Hurwitz and Richard Schiff and Mary Fletcher}, journal={Clinical and diagnostic laboratory immunology}, year={2002}, volume={9 6}, pages={1248-52} }