Quantitative fluorescence imaging of protein diffusion and interaction in living cells

  title={Quantitative fluorescence imaging of protein diffusion and interaction in living cells},
  author={J{\'e}r{\'e}mie Capoulade and Malte Wachsmuth and Lars Hufnagel and Michael Knop},
  journal={Nature Biotechnology},
Diffusion processes and local dynamic equilibria inside cells lead to nonuniform spatial distributions of molecules, which are essential for processes such as nuclear organization and signaling in cell division, differentiation and migration. To understand these mechanisms, spatially resolved quantitative measurements of protein abundance, mobilities and interactions are needed, but current methods have limited capabilities to study dynamic parameters. Here we describe a microscope based on… 
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