Quantitative ex vivo detection of rodent retinal ganglion cells by immunolabeling Brn-3b.

@article{Leahy2004QuantitativeEV,
  title={Quantitative ex vivo detection of rodent retinal ganglion cells by immunolabeling Brn-3b.},
  author={Kathleen M. Leahy and Richard L. Ornberg and Yu Wang and Yanli Zhu and Jeffrey M. Gidday and Jane R. Connor and M. Wax},
  journal={Experimental eye research},
  year={2004},
  volume={79 1},
  pages={131-40}
}
To evaluate the neuroprotective potential of drug candidates to treat human glaucoma, a short-term rodent model of retinal ganglion cell death was employed. Transient ischemia applied to the rodent retina, with subsequent reperfusion for 1-4 weeks, produces an experimental retinal ganglion cell death that is quantifiable. A widely used method to detect viable retinal ganglion cells involves surgical injection of labeling compounds into the superior colliculus of the rodent brain, the retrograde… CONTINUE READING