Quantitative evaluation of reference genes for real-time PCR during in vitro maturation of ovine oocytes.

@article{OConnor2013QuantitativeEO,
  title={Quantitative evaluation of reference genes for real-time PCR during in vitro maturation of ovine oocytes.},
  author={Timothy E. O'Connor and Ian Wilmut and Jane Taylor},
  journal={Reproduction in domestic animals = Zuchthygiene},
  year={2013},
  volume={48 3},
  pages={477-83}
}
Quantitative reverse transcription PCR (RT-qPCR) is a powerful molecular technique that enables gene expression studies to be performed on extremely small samples such as oocytes and pre-implantation embryos. However, the high sensitivity of this technique requires that to prevent bias in expression data interpretation, the reference genes used for normalization are fully validated before use. Difficulties in choosing a reference gene are further compounded by the variable RNA content of the… CONTINUE READING
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