Quantitative evaluation of mitochondrial calcium content in rat cortical neurones following a glutamate stimulus.

@article{Brocard2001QuantitativeEO,
  title={Quantitative evaluation of mitochondrial calcium content in rat cortical neurones following a glutamate stimulus.},
  author={Jacques B Brocard and Michel Tassetto and Ian J. Reynolds},
  journal={The Journal of physiology},
  year={2001},
  volume={531 Pt 3},
  pages={793-805}
}
1. Recent observations showed that a mitochondrial Ca2+ increase is necessary for an NMDA receptor stimulus to be toxic to cortical neurones. In an attempt to determine the magnitude of the Ca2+ fluxes involved in this phenomenon, we used carbonylcyanide-p-(trifluoromethoxy)phenylhydrazone (FCCP), a mitochondrial proton gradient uncoupler, to release mitochondrial free calcium ([Ca2+]m) during and following a glutamate stimulus, and magfura-2 to monitor cytoplasmic free calcium ([Ca2+]c). 2… CONTINUE READING

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