Quantitative evaluation of blood-brain barrier permeability following middle cerebral artery occlusion in rats

  title={Quantitative evaluation of blood-brain barrier permeability following middle cerebral artery occlusion in rats},
  author={Ludmila Belayev and Raul Busto and Weizhao Zhao and Myron D. Ginsberg},
  journal={Brain Research},


It is indicated that quantitative detection of Evans blue extravasation technique is capable of measuring the alterations of BBB permeability in cerebral ischemia in the rat, indicating severe motor disabilities due to cerebral ischemic injury.

Late changes in blood–brain barrier permeability in a rat tMCAO model of stroke detected by gadolinium-enhanced MRI

The results indicate a significant late opening of the blood–brain barrier, which may indicate an optimal time for drug delivery across the BBB, when it is especially suited to drugs targeting delayed processes.

Transient Middle Cerebral Artery Occlusion by Intraluminal Suture: I. Three-Dimensional Autoradiographic Image-Analysis of Local Cerebral Glucose Metabolism—Blood Flow Interrelationships during Ischemia and Early Recirculation

Regression analysis indicated that depressed glucose utilization in a highly reproducible model of transient middle cerebral artery occlusion for 2 hours induced graded LCBF decrements affecting ipsilateral cortical and basal ganglionic regions, and was determined largely by the intensity of antecedent ischemia.

Severe Blood–Brain Barrier Disruption and Surrounding Tissue Injury

Severe vascular disruption, as labeled with high-molecular-weight dextran-fluorescein isothiocyanate leakage, is associated with severe tissue injury and may be useful in further studies of the pathoanatomic mechanisms of vascular disruption-mediated tissue injury.



Transient Blood-Brain Barrier Permeability following Profound Temporary Global Ischaemia: An Experimental Study Using 14C-AIB

  • J. DobbinH. CrockardR. Ross-Russell
  • Biology, Medicine
    Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism
  • 1989
It is concluded that following transient global ischaemia, the BBB may recover rapidly, and there was a significant increase in transfer of AIB across the blood–brain barrier (BBB) after recirculation for up to 6 h, though there was no evidence of protein extravasation as assessed by Evans Blue (EB) dye.

Brain edema and cerebrovascular permeability during cerebral ischemia in rats.

Disruption of the blood-brain barrier to small molecules is accompanied by accumulation of edema fluid during the later stages of ischemia, indicating that opening of the barrier to serum protein is probably related to the resolution of Edema.

Early Changes in Blood Brain Barrier Permeability To Small Molecules After Transient Cerebral Ischemia

Brain unidirectional extraction and flux of leucine were measured simultaneously with cerebral blood flow after transient global cerebral ischemia in the rat to permit an evaluation of blood-brain barrier permeability in the postischemic period independent of alter- ations in CBF at the time of measurement.


Vulnerability of the blood-brain barrier to unilateral intracarotid perfusion with chemical injurious agents was studied with the application of various fluorescent and radioactive tracers to find patterns of its abnormal penetration through the damaged cerebral vessels.

Disruption of blood‐brain barrier following bilateral carotid artery occlusion in spontaneously hypertensive rats. A quantitative study.

The present study was designed to clarify the relationship of cerebral blood flow (CBF) to blood-brain barrier (BBB) in the ischemic brains with or without recirculation, which were produced by

Correlation between cerebral blood flow and histologic changes in a new rat model of middle cerebral artery occlusion.

A new focal ischemia model consisting of unilateral middle cerebral artery occlusion with a silicone rubber cylinder attached to a nylon surgical thread inserted through the internal carotid artery in rats that should be useful in various investigations of the influence of reperfusion on focal ischemic brain injury.

Gross Persistence of Capillary Plasma Perfusion after Middle Cerebral Artery Occlusion in the Rat Brain

  • H. TheilenH. SchröckW. Kuschinsky
  • Biology, Medicine
    Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism
  • 1994
The data show that a drastic reduction of cortical CBF after MCA occlusion is not accompanied by a corresponding amount of nonperfused capillaries, and this condition is not associated with a more extensive lack of capillary perfusion.

Reversible middle cerebral artery occlusion without craniectomy in rats.

Seven of eight rats that underwent permanent occlusion of the MCA had resolving moderately severe neurologic deficits and unilateral infarcts averaging 37.6 +/- 5.5% of the coronal sectional area at 72 hours after the onset of Occlusion.

Reperfusion‐Induced Injury to the Blood‐Brain Barrier After Middle Cerebral Artery Occlusion in Rats

Findings demonstrate that brain infarct and blood-brain barrier disruption are exacerbated after reperfusion in this model of focal ischemia, and that blood- brain barrier disruption may relate to the degree of cerebral blood flow recovery.