Quantitative enzyme activity determination with zeptomole sensitivity by microfluidic gradient-gel zymography.

Abstract

We describe a sensitive zymography technique that utilizes an automated microfluidic platform to report enzyme molecular weight, amount, and activity (including k(cat) and K(m)) from dilute protein mixtures. Calf intestinal alkaline phosphatase (CIP) is examined in detail as a model enzyme system, and the method is also demonstrated for horseradish… (More)
DOI: 10.1021/ac100201z

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Cite this paper

@article{Hughes2010QuantitativeEA, title={Quantitative enzyme activity determination with zeptomole sensitivity by microfluidic gradient-gel zymography.}, author={Alex J Hughes and Amy E Herr}, journal={Analytical chemistry}, year={2010}, volume={82 9}, pages={3803-11} }