Quantitative duplex PCR of Clostridium botulinum types A and B neurotoxin genes.

Abstract

A duplex quantitative polymerase chain reaction (PCR) assay for Clostridium botulinum types A and B was developed. The sensitivity and specificity of the assay were verified by using 6 strains of type A, 7 strains of type B, and 14 genera of 42 non-C. botulinum types A and B strains, including C. botulinum types C, D, E, F, and G. In pure culture, the… (More)

Topics

Cite this paper

@article{Kasai2007QuantitativeDP, title={Quantitative duplex PCR of Clostridium botulinum types A and B neurotoxin genes.}, author={Yoshiaki Kasai and Bon Kimura and Yosuke Tajima and Tateo Fujii}, journal={Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan}, year={2007}, volume={48 1}, pages={19-26} }