Quantitative dissection and stoichiometry determination of the human SET1/MLL histone methyltransferase complexes.

@article{Nuland2013QuantitativeDA,
  title={Quantitative dissection and stoichiometry determination of the human SET1/MLL histone methyltransferase complexes.},
  author={Rick van Nuland and Arne H Smits and Paschalina Pallaki and Pascal W. T. C. Jansen and Michiel Vermeulen and H. Th. Marc Timmers},
  journal={Molecular and cellular biology},
  year={2013},
  volume={33 10},
  pages={2067-77}
}
Methylation of lysine 4 on histone H3 (H3K4) at promoters is tightly linked to transcriptional regulation in human cells. At least six different COMPASS-like multisubunit (SET1/MLL) complexes that contain methyltransferase activity for H3K4 have been described, but a comprehensive and quantitative analysis of these SET1/MLL complexes is lacking. We applied label-free quantitative mass spectrometry to determine the subunit composition and stoichiometry of the human SET1/MLL complexes. We… CONTINUE READING