Quantitative determinations of calmodulin in the supernatant and particulate fractions of mammalian tissues.

@article{Kakiuchi1982QuantitativeDO,
  title={Quantitative determinations of calmodulin in the supernatant and particulate fractions of mammalian tissues.},
  author={S. Kakiuchi and Satoshi Yasuda and Ryuichiro Yamazaki and Yasushi Teshima and K Kanda and R Kakiuchi and Kenji Sobue},
  journal={Journal of biochemistry},
  year={1982},
  volume={92 4},
  pages={1041-8}
}
Although calmodulin is generally regarded as a soluble protein, a considerable amount of calmodulin activity was found to be associated with particulate fractions of mammalian tissues after an extensive washing of the particulate fraction with EGTA. Identity of this particle-bound and EGTA-nonextractable form of calmodulin with soluble calmodulin was established recently (Sobue, K., Yamazaki, R., Yasuda, S., & Kakiuchi, S. (1981) FEBS Lett. 129, 215-219). The particle-associated calmodulin… CONTINUE READING

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