Quantitative determination of vitamin D metabolites in plasma using UHPLC-MS/MS

@inproceedings{Ding2010QuantitativeDO,
  title={Quantitative determination of vitamin D metabolites in plasma using UHPLC-MS/MS},
  author={Shujing Ding and Inez Schoenmakers and Kerry S Jones and Albert Koulman and Ann Prentice and Dietrich A Volmer},
  booktitle={Analytical and bioanalytical chemistry},
  year={2010}
}
Vitamin D is an important determinant of bone health at all ages. The plasma concentrations of 25-hydroxy vitamin D (25-OH D) and other metabolites are used as biomarkers for vitamin sufficiency and function. To allow for the simultaneous determination of five vitamin D metabolites, 25-OH D(3), 25-OH D(2), 24,25-(OH)(2) D(3), 1,25-(OH)(2) D(3), and 1,25-(OH)(2) D(2), in low volumes of human plasma, an assay using ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS… CONTINUE READING
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