Quantitative determination of the mineral distribution in different collagen zones of calcifying tendon using high voltage electron microscopic tomography.


High voltage electron microscopic tomography was used to make the first quantitative determination of the distribution of mineral between different regions of collagen fibrils undergoing early calcification in normal leg tendons of the domestic turkey, Meleagris gallopavo. The tomographic 3-D reconstruction was computed from a tilt series of 61 different… (More)