Quantitative determination of rDNA transcription units in vertebrate cells.

@article{Haaf1991QuantitativeDO,
  title={Quantitative determination of rDNA transcription units in vertebrate cells.},
  author={Thomas Haaf and D. L. Hayman and Michael Schmid},
  journal={Experimental cell research},
  year={1991},
  volume={193 1},
  pages={78-86}
}
The adenosine analogue 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB) unravels the compact nucleoli to necklace-like structures when applied to living cells. The nucleolar beads contain RNA polymerase I (RPI) and argyrophilic proteins, both properties considered to be characteristic of ribosomal gene activity. Each granule is supposed to represent a single transcription unit consisting of an actively transcribing gene and its RPI complex. Indirect immunofluorescence with anti-RPI… CONTINUE READING