Quantitative determination of protein stability and ligand binding using a green fluorescent protein reporter system.

@article{Moreau2010QuantitativeDO,
  title={Quantitative determination of protein stability and ligand binding using a green fluorescent protein reporter system.},
  author={Morgane J J Moreau and Isabelle Morin and Patrick M. Schaeffer},
  journal={Molecular bioSystems},
  year={2010},
  volume={6 7},
  pages={1285-92}
}
Information about the stability of proteins is paramount to determine their optimal storage or reaction conditions. It is also essential to determine protein stability in high-throughput when screening for new or improved functions of proteins obtained from large mutant libraries. In drug discovery programs, monitoring of ligand-induced stabilization effects can be used to identify lead compounds in high-throughput. These studies require expensive biophysical instrumentation and large… CONTINUE READING