Quantitative determination of free-DNA uptake in river bacteria at the single-cell level by in situ rolling-circle amplification.

Abstract

Detection of plasmid DNA uptake in river bacteria at the single-cell level was carried out by rolling-circle amplification (RCA). Uptake of a plasmid containing the green fluorescent protein gene (gfp) by indigenous bacteria from two rivers in Osaka, Japan, was monitored for 506 h using this in situ gene amplification technique with optimized cell… (More)

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