Quantitative determination of α- and γ-2,4-dinitrophenyl-isomers of α,γ-diaminobutyric acid with an automatic amino acid analyzer as a method of studying Nα ⇄ Nγ migration in peptides of α,γ-diaminobutyric acid and polymyxin M

@article{Silaev1966QuantitativeDO,
  title={Quantitative determination of $\alpha$- and $\gamma$-2,4-dinitrophenyl-isomers of $\alpha$,$\gamma$-diaminobutyric acid with an automatic amino acid analyzer as a method of studying N$\alpha$ ⇄ N$\gamma$ migration in peptides of $\alpha$,$\gamma$-diaminobutyric acid and polymyxin M},
  author={A. Silaev and L. Baratova and G. Katrukha},
  journal={Journal of Chromatography A},
  year={1966},
  volume={24},
  pages={61-67}
}
Abstract For a quantitative estimation of the value of Nα ⇄ Nγ migration in inactive polymyxin M and in peptides of α,γ-diaminobutyric acid (α,γ-DAB) a rapid method of separation and quantitative determination of the α- and γ-2,4-dinitrophenyl-derivatives of α,γ-DAB is suggested. Their breakdown during acid hydrolysis can be followed with an automatic amino acid analyzer (a 15-cm column, Amberlite CG-120; 0.30 N Na+-citrate buffer, pH 5.28, 50°; 3.5 h). This method enables the α- and ω-2,4… Expand
5 Citations
Determination of the water-soluble dinitrophenylamino acids by ion-exchange chromatography.
TLDR
Several water-soluble DNP-amino acids may be determined quantitatively by elution from IRC-50 columns with citrate/NaCl buffer by recording of the yellow color in the 360 mμ region. Expand
EM49, a new peptide antibiotic. II. Chemical characterization.
TLDR
It is concluded that EM 49 is a complex of cyclic, homodectic octapeptide antibiotics that are monoacylated with β-hydroxy fatty acids. Expand
The antibiotic edeine. XI: Chemical structure of edeine D
Edeine D is pentapeptide amide produced with other edeines by Bacillus brevis Vm4 strain. This antibiotic is composed of glycine (Gly), isoserine (Ise), 2,3-diaminopropionic acid (A2pr),Expand
A study of the mechanism of polymyxin M inactivation
При изучении механизма инактивации Полимиксина М в О.Iн.NH4OH было обнаружено, что наряду с N α → N γ -ацильной миграцией имеет место и интрамолекулярный аминолиз пептидной связиγ-аминнойExpand

References

SHOWING 1-8 OF 8 REFERENCES
Chromatography of amino acids on sulfonated polystyrene resins.
TLDR
A systematic study has been made of the separations of amino acids by elution analysis on columns of synthetic ion exchange resins, demonstrating that synthetic resins are capable of separating most of the common amino acids from one another. Expand
THE CHEMISTRY OF POLYMYXIN A
The object of this paper is to describe the present state of our knowledge of polymyxin A, which is the agreed name for Lhe antibiotic originally called “Aerosporin” by Ainsworth, Brown, andExpand
CIRCULIN AN ANTIBIOTIC FROM AN ORGANISM RESEMBLING BACILLUS CIRCULANS
The discovery of an antibiotic that is more active against gram-negative than against gram-positive bacteria and that appears to be related to the polymyxinaerosporin type of antibiotics was recentlyExpand
Chemistry and microbiology