Quantitative detection of human tumor necrosis factor α by a resonance raman enzyme-linked immunosorbent assay.

@article{Laing2011QuantitativeDO,
  title={Quantitative detection of human tumor necrosis factor α by a resonance raman enzyme-linked immunosorbent assay.},
  author={Stacey Laing and Aaron Hernandez-Santana and J{\"o}rg Sassmannshausen and Darren Lee Asquith and Iain B. Mcinnes and Karen Faulds and Duncan Graham},
  journal={Analytical chemistry},
  year={2011},
  volume={83 1},
  pages={297-302}
}
Tumor necrosis factor α is an inflammatory cytokine which has been linked with many infectious and inflammatory diseases. Detection and quantification of this key biomarker is commonly achieved by use of an enzyme-linked immunosorbent assay (ELISA). This fundamental technique uses the spectroscopic detection of a chromogen such as 3,3',5,5'-tetramethylbenzidine (TMB). Horseradish peroxidase (HRP), bound to the detection antibody, catalyzes the oxidation of TMB by hydrogen peroxide to generate… CONTINUE READING
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