Quantitative detection of T-cell activation markers by real-time PCR in renal transplant rejection and correlation with histopathologic evaluation.

@article{Sabek2002QuantitativeDO,
  title={Quantitative detection of T-cell activation markers by real-time PCR in renal transplant rejection and correlation with histopathologic evaluation.},
  author={Omaima M Sabek and Mehmet Tevfik Dorak and Malak Kotb and A Osama Gaber and Lillian W. Gaber},
  journal={Transplantation},
  year={2002},
  volume={74 5},
  pages={701-7}
}
BACKGROUND The quest for noninvasive methods to diagnose rejection in solid-organ transplants has been rejuvenated by recent observations that specific cytotoxic T-cell markers are up-regulated during rejection. METHODS We developed a one-step real-time polymerase chain reaction (PCR) method allowing reliable detection of the expression of several T-cell genes within a relatively short period of time. The assay is highly sensitive and reproducible with a wide dynamic range allowing accurate… CONTINUE READING

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