Quantitative detection of Borrelia burgdorferi with a microtiter-based competitive polymerase chain reaction assay.

@article{Germer1999QuantitativeDO,
  title={Quantitative detection of Borrelia burgdorferi with a microtiter-based competitive polymerase chain reaction assay.},
  author={Jeffrey J. Germer and B Ryckmann and Massimo Moro and Erik Hofmeister and Stephen W. Barthold and Linda K Bockenstedt and David H. Persing},
  journal={Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology},
  year={1999},
  volume={4 3},
  pages={185-93}
}
BACKGROUND The current understanding of the inflammation associated with Lyme disease directly involves infection caused by Borrelia burgdorferi within specific target tissues, accompanied by a significant host immunologic component driving the inflammatory process. The measurement of spirochetal tissue burden may thus be useful for studying animal models of Lyme disease pathogenesis. Widely available methods based on the culture of spirochetes from tissues do not provide quantitative… CONTINUE READING