Quantitative detection of Borrelia burgdorferi by real-time PCR.

@article{Pahl1999QuantitativeDO,
  title={Quantitative detection of Borrelia burgdorferi by real-time PCR.},
  author={Andreas Pahl and U K{\"u}hlbrandt and Kieran Brune and Martin Roellinghoff and A. Gabriela Castro Gessner},
  journal={Journal of clinical microbiology},
  year={1999},
  volume={37 6},
  pages={
          1958-63
        }
}
Currently, no easy and reliable methods allowing for the quantification of Borrelia burgdorferi in tissues of infected humans or animals are available. Due to the lack of suitable assays to detect B. burgdorferi CFU and the qualitative nature of the currently performed PCR assays, we decided to exploit the recently developed real-time PCR. This technology measures the release of fluorescent oligonucleotides during the PCR. Flagellin of B. burgdorferi was chosen as the target sequence. A linear… CONTINUE READING

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