Quantitative comparison of pretreatment regimens used to sensitize in situ hybridization using oligonucleotide probes on paraffin-embedded brain tissue.

@article{Oliver1997QuantitativeCO,
  title={Quantitative comparison of pretreatment regimens used to sensitize in situ hybridization using oligonucleotide probes on paraffin-embedded brain tissue.},
  author={Kevin R Oliver and Robert P. Heavens and Dalip J. S. Sirinathsinghji},
  journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society},
  year={1997},
  volume={45 12},
  pages={1707-13}
}
Paraffin embedding of tissue is generally perceived to dramatically reduce RNA detectability. As a consequence, in situ hybridization on paraffin-embedded tissue is largely confined to detection of high-copy RNA species (e.g., viral RNA) and/or to detection using typically more sensitive cDNA probes or riboprobes. In this study, several procedures for in situ hybridization on paraffin-embedded rat tissue using oligonucleotide probes complementary to cellular transcripts were developed and… CONTINUE READING

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